Eyespots (color patterns resembling eyes) are present in many taxa and often have important ecological functions. In some taxa, potential functions of eyespots have not been extensively examined, as is the case with pygmy-owls (Glaucidium spp.), many of which bear a conspicuous eye-like pattern on their nape. Using wooden replicas of Northern Pygmy-Owls (G. gnoma), with and without eyespots, we tested one potential function: whether eyespots affect mobbing behaviors of small forest birds. We measured the orientation of mobbing behaviors (where birds perched and flew at the model) and intensity of mobbing bouts (duration of bouts and number of mobbing individuals). Eyespots affected the most proximal behavior, close passing (flights directly at the model, resulting in an approach within 0.5 m). When eyespots were present, mobbers shifted away from eyespots, providing the first empirical evidence for a link between eyespots and avian mobbing.